4CGC
Crystal structure of the trimerization domain of human EML4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-29 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 67.491, 67.491, 50.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.723 - 2.901 |
| R-factor | 0.2687 |
| Rwork | 0.268 |
| R-free | 0.29150 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.020 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SCALA |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.490 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.130 | 1.250 |
| Number of reflections | 2864 | |
| <I/σ(I)> | 13.3 | 3.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.4 | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 100 MM TRIS/HCL PH 8.5, 30% PEG 4000, 200 MM MGCL2 |
