4CFY
SAVINASE CRYSTAL STRUCTURES FOR COMBINED SINGLE CRYSTAL DIFFRACTION AND POWDER DIFFRACTION ANALYSIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-07 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.230, 61.820, 75.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.880 - 1.170 |
| R-factor | 0.09368 |
| Rwork | 0.092 |
| R-free | 0.11886 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1svn |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.882 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 23.880 |
| High resolution limit [Å] | 1.170 |
| Rmerge | 0.420 |
| Number of reflections | 58833 |
| <I/σ(I)> | 39.8 |
| Completeness [%] | 78.2 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20 MG/ML PROTEIN, 4% PEG 4000, 0.33 M NACL, 1.5 MM CACL2, 18 MM CITRATE BUFFER PH 6.0 |
