4CF5
Mutagenesis of a Rhodobacteraceae L-haloacid dehalogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 287 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.320, 67.740, 284.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.550 - 2.340 |
| R-factor | 0.17929 |
| Rwork | 0.176 |
| R-free | 0.25028 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yml |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.331 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.550 | 2.400 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.100 | 0.620 |
| Number of reflections | 36556 | |
| <I/σ(I)> | 14.6 | 3 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 6.5 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | pH 8 |
