4CEV
ARGINASE FROM BACILLUS CALDEVELOX, L-ORNITHINE COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 1997-01 |
Detector | RIGAKU |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 106.600, 277.300, 139.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.700 |
R-factor | 0.203 * |
Rwork | 0.203 |
R-free | 0.27400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | REFINED MODEL FOR THE PH 8.5 NATIVE STRUCTURE: 2CEV |
RMSD bond length | 0.010 |
RMSD bond angle | 1.420 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.097 | 0.219 |
Total number of observations | 161259 * | |
Number of reflections | 53853 | |
<I/σ(I)> | 10.1 | 3 |
Completeness [%] | 93.5 | 82.4 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | RESERVOIR SOLUTION 28% MONOMETHYLPEG 2000, 5 MM MNCL2, 10 MM GUANIDINE HYDROCHLORIDE, 10 MM D/L-ORNITHINE,IN 0.05 M BISTRISPROPANE/HCL,PH 8.5 27 MG/ML PROTEIN, 10 MM MOPSm, PH 7.5 , VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | mmPEG2000 | 28 (%) | |
2 | 1 | reservoir | guanidine hydrochloride | 10 (mM) | |
3 | 1 | reservoir | 5 (mM) | ||
4 | 1 | reservoir | BTP/HCl | 50 (mM) | |
5 | 1 | reservoir | D/L-ornithine | 10 (mM) |