4CEL
ACTIVE-SITE MUTANT D214N DETERMINED AT PH 6.0 WITH NO LIGAND BOUND IN THE ACTIVE SITE
Experimental procedure
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1994-09-15 |
Detector | RIGAKU |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 84.000, 86.200, 111.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.500 - 2.200 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.23900 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 * |
Data reduction software | DENZO |
Data scaling software | CCP4 |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.600 | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.067 | 0.221 |
Total number of observations | 143220 * | |
Number of reflections | 41627 | |
<I/σ(I)> | 9.7 | 3.5 |
Completeness [%] | 99.6 | 98.1 |
Redundancy | 3.4 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 | 4.5 MM MES PH 6.0, 9% PEG 6000, 4.5 MM CACL2 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4 (mg/ml) | |
2 | 1 | drop | MES | 4.5 (mM) | |
3 | 1 | drop | PEG6000 | 9 (%(w/v)) | |
4 | 1 | drop | 4.5 (mM) | ||
5 | 1 | reservoir | 0.2 (M) |