4CE2
Hsp90 N-terminal domain bound to macrolactam analogues of radicicol.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-04-28 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 74.320, 74.320, 110.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.460 - 2.380 |
R-factor | 0.19527 |
Rwork | 0.193 |
R-free | 0.23936 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ah6 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.848 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.460 | 2.450 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmerge | 0.110 | 0.800 |
Number of reflections | 12939 | |
<I/σ(I)> | 14.2 | 2.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 8.5 | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | pH 7 |