4CE1
Hsp90 N-terminal domain bound to macrolactam analogues of radicicol.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-28 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 74.160, 74.160, 110.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.440 - 2.010 |
| R-factor | 0.2087 |
| Rwork | 0.206 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ah6 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.935 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.440 | 2.060 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.060 | 0.720 |
| Number of reflections | 21192 | |
| <I/σ(I)> | 20.4 | 3.5 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 8.5 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
