4CDO
Crystal structure of PQBP1 bound to spliceosomal U5-15kD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Collection date | 2011-12-07 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.360, 53.700, 66.190 |
Unit cell angles | 90.00, 89.95, 90.00 |
Refinement procedure
Resolution | 33.095 - 2.500 |
R-factor | 0.1895 |
Rwork | 0.187 |
R-free | 0.23820 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.054 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.820 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.160 | 0.640 |
Number of reflections | 14640 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |