4CDH

Crystallographic structure of the Human Igg1 alpha 2-6 sialilated Fc-Fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Spacegroup name	P 21 21 21
Unit cell lengths	49.590, 80.150, 140.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.100 - 2.300
R-factor	0.23647
Rwork	0.234
R-free	0.28215
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.016
RMSD bond angle	2.069
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.100
High resolution limit [Å]	2.300
Rmerge	0.090
Number of reflections	24279
<I/σ(I)>	11.7
Completeness [%]	100.0
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	CRYSTALLIZATION TRIALS WERE PERFORMED AT 18 C BY THE SITTING DROP VAPOUR DIFFUSION METHOD. CRYSTALS GROWN BY MIXING 1 UL OF FC-SIAL (9 MG/ML IN PBS) WITH 3 UL OF PRECIPITANT SOLUTION CONSISTING OF 0.1M HEPES PH7, 16% PEG 6000, VAPOR DIFFUSION, TEMPERATURE 298K