4CD5
The structure of GH26 beta-mannanase CjMan26C from Cellvibrio japonicus in complex with ManMIm
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-07 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 84.507, 84.507, 244.941 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 73.190 - 1.100 |
R-factor | 0.15273 |
Rwork | 0.152 |
R-free | 0.17213 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vx7 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.549 |
Data reduction software | xia2 (IMPLEMENTATION OF XDS) |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.000 | 1.120 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmerge | 0.100 | |
Number of reflections | 208589 | |
<I/σ(I)> | 11.7 | 1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 15.3 | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | 100MM SODIUM CITRATE PH 5.6, 15% PEG 3000 |