4CD4
The structure of GH26 beta-mannanase CjMan26C from Cellvibrio japonicus in complex with ManIFG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-17 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 84.518, 84.518, 244.491 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 73.190 - 1.200 |
| R-factor | 0.11241 |
| Rwork | 0.111 |
| R-free | 0.13385 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vx7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.519 |
| Data reduction software | xia2 (IMPLEMENTATION OF XDS) |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0033) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.460 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.060 | 0.690 |
| Number of reflections | 142150 | |
| <I/σ(I)> | 21.9 | 0.8 |
| Completeness [%] | 88.8 | 20.8 |
| Redundancy | 14.8 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 100 MM SODIUM CITRATE PH 5.6, 15% PEG 3000 |
