4CCV
Crystal structure of histidine-rich glycoprotein N2 domain reveals redox activity at an interdomain disulfide bridge: Implications for the regulation of angiogenesis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-26 |
Detector | DECTRIS PILATUS 2M |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 77.138, 77.138, 69.360 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.710 - 1.930 |
R-factor | 0.18671 |
Rwork | 0.185 |
R-free | 0.22524 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.017 |
RMSD bond angle | 2.019 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.710 | 2.000 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.050 | 0.330 |
Number of reflections | 17163 | |
<I/σ(I)> | 11.3 | 2.1 |
Completeness [%] | 93.2 | 69.2 |
Redundancy | 4.1 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 22.5% PEG MME 5000, 0.073M POTASSIUM SODIUM TARTRATE, 0.1M MES PH6.5 |