4CC7
Crystal structure of the sixth or C-terminal SH3 domain of human Tuba in complex with proline-rich peptides of N-WASP. Space group P41
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-12-18 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 41 |
Unit cell lengths | 88.570, 88.570, 69.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.590 - 1.970 |
R-factor | 0.17798 |
Rwork | 0.176 |
R-free | 0.21459 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zuu |
RMSD bond length | 0.019 |
RMSD bond angle | 2.038 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.090 |
High resolution limit [Å] | 1.970 | 1.970 |
Rmerge | 0.050 | 0.710 |
Number of reflections | 38387 | |
<I/σ(I)> | 20.7 | 2.6 |
Completeness [%] | 99.8 | 99.2 |
Redundancy | 6 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 3 M NACL, 0.1 M BIS-TRIS PH 5.5 |