4CBX
Crystal structure of Plasmodium berghei actin II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-17 |
| Detector | PLATINUM135 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.248, 60.908, 75.518 |
| Unit cell angles | 90.00, 97.24, 90.00 |
Refinement procedure
| Resolution | 31.907 - 2.200 |
| R-factor | 0.1975 |
| Rwork | 0.196 |
| R-free | 0.22750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p8z |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.670 |
| Data reduction software | PROTEUM2 |
| Data scaling software | PROTEUM2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.000 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.160 | 0.790 |
| Number of reflections | 29458 | |
| <I/σ(I)> | 5.6 | 0.9 |
| Completeness [%] | 99.4 | 95.2 |
| Redundancy | 3.6 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 18% (W/V) PEG 8000, 0.2 M AMMONIUM SULFATE, 0.1 M BIS-TRIS PROPANE PH 6.5 |
