4CBK
The c-ring ion binding site of the ATP synthase from Bacillus pseudofirmus OF4 is adapted to alkaliphilic cell physiology
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-03 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.790, 96.750, 118.330 |
Unit cell angles | 90.00, 105.38, 90.00 |
Refinement procedure
Resolution | 40.004 - 2.420 |
R-factor | 0.1966 |
Rwork | 0.195 |
R-free | 0.22830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x2v |
RMSD bond length | 0.004 |
RMSD bond angle | 0.793 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.500 |
High resolution limit [Å] | 2.420 | 2.420 |
Rmerge | 0.170 | 0.970 |
Number of reflections | 60954 | |
<I/σ(I)> | 12.33 | 1.84 |
Completeness [%] | 99.3 | 99 |
Redundancy | 4.53 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 9 | pH 9 |