4CBH
Pestivirus NS3 helicase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.070, 144.440, 118.690 |
| Unit cell angles | 90.00, 92.94, 90.00 |
Refinement procedure
| Resolution | 72.220 - 2.510 |
| R-factor | 0.2048 |
| Rwork | 0.203 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cbg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.280 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.13.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.340 | 2.580 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Rmerge | 0.080 | 0.480 |
| Number of reflections | 71319 | |
| <I/σ(I)> | 14.8 | 1.5 |
| Completeness [%] | 88.8 | 49.6 |
| Redundancy | 3.6 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M NA-CACODYLATE, 0.2 M MAGNESIUM ACETATE, 12% PEG 8000, pH 6.5 |
