4CBH
Pestivirus NS3 helicase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-13 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.070, 144.440, 118.690 |
Unit cell angles | 90.00, 92.94, 90.00 |
Refinement procedure
Resolution | 72.220 - 2.510 |
R-factor | 0.2048 |
Rwork | 0.203 |
R-free | 0.23200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4cbg |
RMSD bond length | 0.010 |
RMSD bond angle | 1.280 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | BUSTER (2.13.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.340 | 2.580 |
High resolution limit [Å] | 2.510 | 2.510 |
Rmerge | 0.080 | 0.480 |
Number of reflections | 71319 | |
<I/σ(I)> | 14.8 | 1.5 |
Completeness [%] | 88.8 | 49.6 |
Redundancy | 3.6 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 0.1 M NA-CACODYLATE, 0.2 M MAGNESIUM ACETATE, 12% PEG 8000, pH 6.5 |