4CB8
Structural and mutational analysis reveals that CTNNBL1 binds NLSs in a manner distinct from that of its closest armadillo-relative, karyopherin alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.101, 92.686, 121.685 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.210 - 2.900 |
| R-factor | 0.19511 |
| Rwork | 0.191 |
| R-free | 0.26861 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.432 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.100 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.070 | 0.490 |
| Number of reflections | 15836 | |
| <I/σ(I)> | 14.6 | 3.3 |
| Completeness [%] | 97.6 | 99 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 26% PEG 3350, 0.2M LI2SO4, 0.1M TRIS-HCL PH 8.5 |
