4CAH
Structure of inner DysF domain of human dysferlin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-05 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 75.953, 75.953, 75.953 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.707 - 1.901 |
| R-factor | 0.1781 |
| Rwork | 0.177 |
| R-free | 0.19080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2k2o |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.764 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MrBUMP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.700 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.040 | 0.670 |
| Number of reflections | 11803 | |
| <I/σ(I)> | 30.5 | 3.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.8 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.04M POTASSIUM DIHYDROGEN PHOSPHATE, 16% PEG 8000, 20% GLYCEROL, 0.2M NABR, pH 7.5 |
