4CA0
Structural Basis for the microtubule binding of the human kinetochore Ska complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-06-21 |
Detector | MAR scanner 345 mm plate |
Spacegroup name | P 32 |
Unit cell lengths | 47.180, 47.180, 116.500 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.443 - 2.259 |
R-factor | 0.2292 |
Rwork | 0.225 |
R-free | 0.26750 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.117 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 2.370 | 2.370 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.050 | 0.310 |
Number of reflections | 13570 | |
<I/σ(I)> | 17.3 | 3.6 |
Completeness [%] | 98.5 | 90.1 |
Redundancy | 4.5 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 24% PEG 1500 AND 20% GLYCEROL, pH 7 |