4CA0
Structural Basis for the microtubule binding of the human kinetochore Ska complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-06-21 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 32 |
| Unit cell lengths | 47.180, 47.180, 116.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.443 - 2.259 |
| R-factor | 0.2292 |
| Rwork | 0.225 |
| R-free | 0.26750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.117 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.370 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.050 | 0.310 |
| Number of reflections | 13570 | |
| <I/σ(I)> | 17.3 | 3.6 |
| Completeness [%] | 98.5 | 90.1 |
| Redundancy | 4.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 24% PEG 1500 AND 20% GLYCEROL, pH 7 |
