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4CA0

Structural Basis for the microtubule binding of the human kinetochore Ska complex

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU MICROMAX-007
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date2012-06-21
DetectorMAR scanner 345 mm plate
Spacegroup nameP 32
Unit cell lengths47.180, 47.180, 116.500
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution15.443 - 2.259
R-factor0.2292
Rwork0.225
R-free0.26750
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.008
RMSD bond angle1.117
Data reduction softwareMOSFLM
Data scaling softwareSCALA
Phasing softwarePHENIX
Refinement softwarePHENIX ((PHENIX.REFINE))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]2.3702.370
High resolution limit [Å]2.2502.250
Rmerge0.0500.310
Number of reflections13570
<I/σ(I)>17.33.6
Completeness [%]98.590.1
Redundancy4.54
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1724% PEG 1500 AND 20% GLYCEROL, pH 7

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