4C9P
Structure of camphor bound T260A mutant of CYP101D1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-28 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 151.702, 151.702, 196.644 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.581 - 1.799 |
| R-factor | 0.1699 |
| Rwork | 0.169 |
| R-free | 0.19380 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.192 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.580 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.080 | 1.070 |
| Number of reflections | 123065 | |
| <I/σ(I)> | 22.3 | 3 |
| Completeness [%] | 99.8 | 98.6 |
| Redundancy | 16.2 | 14.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.2 | 100 MM TRIS, PH 8.2, 1.6M AMMONIUM SULPHATE |
