4C9D
Cas6 (TTHB231) product complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-14 |
| Detector | ADSC |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 75.220, 75.220, 308.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.083 - 3.000 |
| R-factor | 0.246 |
| Rwork | 0.244 |
| R-free | 0.29070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c98 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.942 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.080 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.130 | 0.780 |
| Number of reflections | 18676 | |
| <I/σ(I)> | 16.6 | 2.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.7 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1M TRIS (PH 8.5), 13% (W/V) PEG 65 20000 |
