4C9C
Crystal Structure of the Strawberry Pathogenesis-Related 10 (PR-10) Fra a 1E protein (Form A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-16 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.020, 74.420, 84.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.030 - 2.200 |
| R-factor | 0.20788 |
| Rwork | 0.206 |
| R-free | 0.25011 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3e85 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.863 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.030 | 36.030 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.030 | |
| Number of reflections | 24107 | |
| <I/σ(I)> | 14 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M AMMONIUM SULFATE, 0.1 M SODIUM CACODYLATE PH 6.5, 15% PEG 8000 |
