4C92

Crystal structure of the yeast Lsm1-7 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-23
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	61.796, 90.570, 68.462
Unit cell angles	90.00, 100.80, 90.00

Refinement procedure

Resolution	53.993 - 2.299
R-factor	0.2133
Rwork	0.211
R-free	0.25850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2Y9A 3bw1 4emk
RMSD bond length	0.008
RMSD bond angle	1.223
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.570	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.050	0.410
Number of reflections	62699
<I/σ(I)>	13.5	3.1
Completeness [%]	99.3	96.5
Redundancy	2.1	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				100 MM MES PH 6.0, 40% MPD