4C8Y
Cas6 (TTHA0078) substrate mimic complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.070, 83.300, 73.440 |
| Unit cell angles | 90.00, 101.41, 90.00 |
Refinement procedure
| Resolution | 71.988 - 1.800 |
| R-factor | 0.192 |
| Rwork | 0.191 |
| R-free | 0.21570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | TTCAS6B STRUCTURE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.114 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.700 |
| Number of reflections | 46544 | |
| <I/σ(I)> | 15.5 | 1.9 |
| Completeness [%] | 98.8 | 97.8 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M BIS-TRIS PROPANE (PH 6.5), 18% (W/V) PEG 335, 0.3M SODIUM SULFATE |
