4C8H
Crystal structure of the C-terminal region of yeast Ctf4, selenomethionine protein.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-12 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 107.294, 118.428, 155.697 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.188 - 2.691 |
| R-factor | 0.1913 |
| Rwork | 0.190 |
| R-free | 0.22480 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.645 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.190 | 2.770 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.230 | 3.380 |
| Number of reflections | 55547 | |
| <I/σ(I)> | 17.4 | 1.1 |
| Completeness [%] | 99.5 | 94.5 |
| Redundancy | 26.7 | 23.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M TRI-SODIUM CITRATE PH 6.2, 7-9% PEG 8000 |
