4C7U
Crystal structure of manganese superoxide dismutase from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-20 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.952, 59.181, 107.980 |
| Unit cell angles | 90.55, 90.51, 89.85 |
Refinement procedure
| Resolution | 53.985 - 1.951 |
| R-factor | 0.1729 |
| Rwork | 0.173 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3AK2 1GV3 1IX9 1LUW 1COJ |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.980 | 2.070 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.070 | 0.570 |
| Number of reflections | 98683 | |
| <I/σ(I)> | 7.64 | 1.09 |
| Completeness [%] | 92.9 | 92.3 |
| Redundancy | 1.74 | 1.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2 M SODIUM FORMATE, 20 % PEG 3350, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 277 K |
