4C6A
High Resolution Structure of the Nucleoside diphosphate kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-16 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 71.700, 71.700, 106.780 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.840 - 1.250 |
| R-factor | 0.1638 |
| Rwork | 0.163 |
| R-free | 0.17210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fkb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.840 | 1.280 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.070 | 0.750 |
| Number of reflections | 45344 | |
| <I/σ(I)> | 46.1 | 10.6 |
| Completeness [%] | 99.7 | 98.9 |
| Redundancy | 21 | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 26% PEG 1000, 100MM TRIS PH7.5 |
