4C69
ATP binding to murine voltage-dependent anion channel 1 (mVDAC1).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-30 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 100.152, 58.369, 66.545 |
Unit cell angles | 90.00, 99.25, 90.00 |
Refinement procedure
Resolution | 19.497 - 2.277 |
R-factor | 0.2066 |
Rwork | 0.204 |
R-free | 0.26110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3emn |
RMSD bond length | 0.007 |
RMSD bond angle | 1.109 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.040 | 0.700 |
Number of reflections | 16915 | |
<I/σ(I)> | 23.13 | 2.21 |
Completeness [%] | 99.3 | 99.2 |
Redundancy | 3.64 | 3.62 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 18-20% MPD, 0.1 M TRISHCL (PH 8.5), 10% PEG400 |