4C64
ULTRA HIGH RESOLUTION DICKERSON-DREW DODECAMER B-DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-08-05 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.149, 39.840, 65.598 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.322 - 1.320 |
| R-factor | 0.1426 |
| Rwork | 0.139 |
| R-free | 0.18230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bna |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.087 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.320 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.030 | 0.220 |
| Number of reflections | 16471 | |
| <I/σ(I)> | 32.1 | 6.1 |
| Completeness [%] | 98.0 | 95.9 |
| Redundancy | 6.3 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 40% MPD, SPERMINE, MAGNESIUM CHLORIDE, SODIUM CACODYLATE PH 7.5 |
