4C5F
Structure of Lytic Transglycosylase MltC from Escherichia coli at 2.3 A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-05 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.865, 114.651, 61.404 |
| Unit cell angles | 90.00, 93.47, 90.00 |
Refinement procedure
| Resolution | 45.657 - 2.340 |
| R-factor | 0.1946 |
| Rwork | 0.191 |
| R-free | 0.25550 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.260 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.660 | 2.460 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.120 | 0.620 |
| Number of reflections | 28559 | |
| <I/σ(I)> | 8.64 | 1.8 |
| Completeness [%] | 97.2 | 84.2 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 18% PEG 3350, 0.2M TRIAMMONIUM CITRATE PH 7.5 |
