4C4W
Structure of a rare, non-standard sequence k-turn bound by L7Ae protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-10 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 135.892, 155.545, 146.874 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.456 - 2.950 |
| R-factor | 0.1692 |
| Rwork | 0.167 |
| R-free | 0.21070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1URN AND 4BW0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.174 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.460 | 3.060 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.110 | 0.750 |
| Number of reflections | 31598 | |
| <I/σ(I)> | 12.9 | 2 |
| Completeness [%] | 95.5 | 93.7 |
| Redundancy | 6.8 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 100 MM TRISHCL 8.5 AND 2.0 M MONO-AMMONIUM DIHYDROGEN PHOSPHATE |
