4C4Q
Cryo-EM map of the CSFV IRES in complex with the small ribosomal 40S subunit and DHX29
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 120 |
| Cs (mm) | 2.26 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
| Number of Images | 2000 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 8.5 |
| Software | RELION,SPIDER |
| Symmetry | C1 |
