4C47
Salmonella enterica trimeric lipoprotein SadB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-29 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | H 3 |
| Unit cell lengths | 118.450, 118.450, 159.180 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.520 - 2.448 |
| R-factor | 0.2061 |
| Rwork | 0.204 |
| R-free | 0.24200 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.742 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.500 | 2.600 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.040 | 0.680 |
| Number of reflections | 30586 | |
| <I/σ(I)> | 19.2 | 1.86 |
| Completeness [%] | 99.7 | 99.2 |
| Redundancy | 4.04 | 3.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | SITTING DROP, TEMPERATURE 294K, RESERVOIR SOLUTION CONTAINING 0.2 M LITHIUM SULFATE, 0.1 M TRIS PH 8.5, 20% (W/V) PEG 4000 |
