4C46
ANDREI-N-LVPAS fused to GCN4 adaptors
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-08-25 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.000, 41.600, 64.140 |
Unit cell angles | 90.00, 95.48, 90.00 |
Refinement procedure
Resolution | 36.640 - 1.950 |
R-factor | 0.23962 |
Rwork | 0.238 |
R-free | 0.27543 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gcm |
RMSD bond length | 0.016 |
RMSD bond angle | 1.717 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.600 | 2.070 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.060 | 0.580 |
Number of reflections | 16104 | |
<I/σ(I)> | 11.3 | 1.94 |
Completeness [%] | 98.7 | 97.5 |
Redundancy | 3.28 | 3.33 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | SITTING DROP, PROTEIN SOLUTION 50 MM NACL AND 50 MM SODIUM ACETATE PH 4.0, RESERVOUR SOLUTION 0.1 M HEPES PH 7.5 1.75 M SODIUM BROMIDE |