4C46
ANDREI-N-LVPAS fused to GCN4 adaptors
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-25 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.000, 41.600, 64.140 |
| Unit cell angles | 90.00, 95.48, 90.00 |
Refinement procedure
| Resolution | 36.640 - 1.950 |
| R-factor | 0.23962 |
| Rwork | 0.238 |
| R-free | 0.27543 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gcm |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.717 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.600 | 2.070 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.060 | 0.580 |
| Number of reflections | 16104 | |
| <I/σ(I)> | 11.3 | 1.94 |
| Completeness [%] | 98.7 | 97.5 |
| Redundancy | 3.28 | 3.33 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | SITTING DROP, PROTEIN SOLUTION 50 MM NACL AND 50 MM SODIUM ACETATE PH 4.0, RESERVOUR SOLUTION 0.1 M HEPES PH 7.5 1.75 M SODIUM BROMIDE |
