4C44

Crystal Structure of Truncated Plant Hemoglobin from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR-H
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-04-12
Detector	MAR scanner 345 mm plate
Spacegroup name	P 43 3 2
Unit cell lengths	122.085, 122.085, 122.085
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.200 - 2.650
R-factor	0.20519
Rwork	0.203
R-free	0.25210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ngk
RMSD bond length	0.009
RMSD bond angle	1.440
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.650	2.650
Rmerge	0.090	0.640
Number of reflections	9881
<I/σ(I)>	12.24	2
Completeness [%]	100.0	100
Redundancy	5.68	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				1.2M SODIUM POTASSIUM TARTARATE TETRAHYDRATE, 0.1M TRIS PH 8.5