4C44
Crystal Structure of Truncated Plant Hemoglobin from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-04-12 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 122.085, 122.085, 122.085 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.200 - 2.650 |
| R-factor | 0.20519 |
| Rwork | 0.203 |
| R-free | 0.25210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ngk |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.440 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.090 | 0.640 |
| Number of reflections | 9881 | |
| <I/σ(I)> | 12.24 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.68 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.2M SODIUM POTASSIUM TARTARATE TETRAHYDRATE, 0.1M TRIS PH 8.5 |
