4C40
The molecular recognition of kink turn structure by the L7Ae class of proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-22 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 70.250, 70.250, 47.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.500 - 2.200 |
| Rwork | 0.220 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bw0 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.996 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.540 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.090 | 0.760 |
| Number of reflections | 3827 | |
| <I/σ(I)> | 15.6 | 3.17 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.2 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 3.5M SODIUM FORMATE, 0.1M SODIUM ACETATE PH 4.0 |
