4C3W
Vanadium(IV)-Picolinate Complexed with Lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.120, 78.120, 37.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.940 - 1.280 |
| R-factor | 0.14596 |
| Rwork | 0.144 |
| R-free | 0.17492 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 193l |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.582 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.670 | 1.300 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.120 | 0.690 |
| Number of reflections | 30287 | |
| <I/σ(I)> | 7.3 | 2.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.4 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 4-8% (M/V) NACL IN 0.1 M ACETATE BUFFER PH 4.5. PROTEIN STOCK CONCENTRATION OF 50 MG/ML. HANGING DROP VAPOR DIFFUSION METHOD, 293K |
