4C3M
Structure of wildtype PII from S. elongatus at medium resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 73.418, 73.418, 95.623 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 63.582 - 2.149 |
| R-factor | 0.2111 |
| Rwork | 0.210 |
| R-free | 0.23470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4aff |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.146 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.000 | 2.280 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.070 | 0.570 |
| Number of reflections | 16722 | |
| <I/σ(I)> | 13.7 | 2.4 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 13.7 | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100 MM HEPES, 20% PEG8000, PH 7.5 |
