4C3K
Structure of mixed PII-ADP complexes from S. elongatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 61 |
| Unit cell lengths | 122.463, 122.463, 80.476 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.730 - 3.099 |
| R-factor | 0.2169 |
| Rwork | 0.214 |
| R-free | 0.26940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4aff |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.038 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 3.290 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.130 | 0.760 |
| Number of reflections | 11089 | |
| <I/σ(I)> | 8.8 | 1.8 |
| Completeness [%] | 87.8 | 90 |
| Redundancy | 3.2 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20% PEG4000, 100 MM TRIS, PH 8.5 |
