4C3D
HRSV M2-1, P422 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-31 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 4 2 2 |
| Unit cell lengths | 102.275, 102.275, 90.993 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.740 - 2.520 |
| R-factor | 0.21351 |
| Rwork | 0.211 |
| R-free | 0.26323 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.460 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.740 | 2.610 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.100 | 0.650 |
| Number of reflections | 16695 | |
| <I/σ(I)> | 14.8 | 3.8 |
| Completeness [%] | 98.9 | 91.2 |
| Redundancy | 30 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10 | 30 % (V/V) PEG 400, 0.01 M CDCL2, 0.1 M CAPS PH 10 |
