4C3B
HRSV M2-1, P21 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-16 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.853, 141.616, 141.694 |
| Unit cell angles | 90.00, 96.14, 90.00 |
Refinement procedure
| Resolution | 140.880 - 2.950 |
| R-factor | 0.20787 |
| Rwork | 0.205 |
| R-free | 0.25458 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.523 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 140.880 | 3.010 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.190 | 1.380 |
| Number of reflections | 75535 | |
| <I/σ(I)> | 14.4 | 2.3 |
| Completeness [%] | 99.5 | 98.2 |
| Redundancy | 15 | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10 | 30 % (V/V) PEG 400, 0.1 M CAPS PH 10 |
