4C33
PKA-S6K1 Chimera Apo
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.720, 75.200, 80.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 17.010 - 1.700 |
R-factor | 0.1578 |
Rwork | 0.156 |
R-free | 0.18710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gfc |
RMSD bond length | 0.010 |
RMSD bond angle | 0.940 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.010 | 1.770 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.050 | 0.190 |
Number of reflections | 39431 | |
<I/σ(I)> | 11.4 | 3.8 |
Completeness [%] | 82.5 | 50.8 |
Redundancy | 3.2 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.4 | 18 MG/ML PROTEIN IN 25MM MES-BIS-TRIS PH 6.5, 75MM LICL, 0.1MM EDTA, 1MM MEGA-8, 1MM DTT AND 1MM PKI PEPTIDE MIXED 1:1 WITH 8-16% AQUEOUS METHANOL |