4C2X
Human N-myristoyltransferase isoform 2 (NMT2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-07-11 |
Detector | DECTRIS PILATUS |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.700, 72.320, 114.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.170 - 2.330 |
R-factor | 0.22173 |
Rwork | 0.218 |
R-free | 0.28812 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3iu1 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.672 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0033) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.000 | 2.410 |
High resolution limit [Å] | 2.330 | 2.330 |
Rmerge | 0.060 | 0.640 |
Number of reflections | 18414 | |
<I/σ(I)> | 15.2 | 2.8 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.3 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 25% PEG 3350, 0.2M KCL, 100 MM NA CITRATE PH 5.5 |