4C2T
Crystal structure of full length Deinococcus radiodurans UvrD in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.457, 390.044, 71.559 |
| Unit cell angles | 90.00, 106.13, 90.00 |
Refinement procedure
| Resolution | 46.147 - 3.997 |
| R-factor | 0.2477 |
| Rwork | 0.246 |
| R-free | 0.27130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | DIFFERENT CRYSTAL FORM OF DRUVRD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.075 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.100 | 4.220 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.110 | 0.660 |
| Number of reflections | 30051 | |
| <I/σ(I)> | 10.1 | 2.3 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 4.4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 16-22% PEG 3350, 0.1 M BIS-TRIS PROPANE PH 6.5-7.5, 0.1-0.3 M NA-NITRATE |
