4C2E
Crystal structure of the protease CtpB(S309A) present in a resting state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.052, 72.687, 79.569 |
| Unit cell angles | 117.09, 90.45, 102.71 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.1824 |
| Rwork | 0.182 |
| R-free | 0.19120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c2c |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.280 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 | 0.380 |
| Number of reflections | 93531 | |
| <I/σ(I)> | 17.8 | 3.6 |
| Completeness [%] | 96.7 | 95.4 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10% ISOPROPANOL, 0.1 M IMIDAZOL PH 8.0 |
