4C25
L-fuculose 1-phosphate aldolase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-01 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | I 4 |
Unit cell lengths | 113.490, 113.490, 41.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 80.250 - 2.030 |
R-factor | 0.22617 |
Rwork | 0.223 |
R-free | 0.27799 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fua |
RMSD bond length | 0.012 |
RMSD bond angle | 1.431 |
Data reduction software | xia2 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.140 |
High resolution limit [Å] | 2.030 | 2.030 |
Rmerge | 0.120 | 0.250 |
Number of reflections | 15800 | |
<I/σ(I)> | 6.5 | 3.1 |
Completeness [%] | 97.7 | 98.1 |
Redundancy | 4.5 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | SPFCSA AT A CONCENTRATION OF 14 MG PER ML, BUFFERED IN 20 MM TRIS-HCL (PH 8.0) AND 5 MM DITHIOTHREITOL (DTT) WAS CRYSTALLIZED BY MIXING EQUAL VOLUMES WITH A SOLUTION OF 4% (V PER V) GLYCEROL, 1.50 M AMMONIUM SULPHATE, 100 MM TRIS-HCL (PH 8.5) AND 8 MM ZINC CHLORIDE. |