4C1Y
Crystal Structure of Fucose binding lectin from Aspergillus Fumigatus (AFL) in complex with b-methylfucoside
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-21 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.211, 90.335, 189.168 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.140 - 2.230 |
R-factor | 0.24611 |
Rwork | 0.243 |
R-free | 0.31339 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4agi |
RMSD bond length | 0.012 |
RMSD bond angle | 1.495 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.290 | 2.290 |
High resolution limit [Å] | 2.230 | 2.230 |
Rmerge | 0.130 | 0.350 |
Number of reflections | 56864 | |
<I/σ(I)> | 6.14 | |
Completeness [%] | 99.0 | 97.8 |
Redundancy | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.2M CACL2, 25% PEG4K, 0.1M TRIS, PH 8.0 |