4C1U
Structure of the xylo-oligosaccharide specific solute binding protein from Bifidobacterium animalis subsp. lactis Bl-04 in complex with arabinoxylobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 67.470, 96.630, 56.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.155 - 2.000 |
| R-factor | 0.1556 |
| Rwork | 0.153 |
| R-free | 0.20460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zkk |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.765 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.200 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.080 |
| Number of reflections | 25178 |
| <I/σ(I)> | 15.6 |
| Completeness [%] | 98.6 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M TRIS PH 7.0, 40% PEG 300, 5% PEG 1500 |
