4C1S
Glycoside hydrolase family 76 (mannosidase) Bt3792 from Bacteroides thetaiotaomicron VPI-5482
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-14 |
| Detector | MARRESEARCH MX-300HE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.100, 44.420, 103.050 |
| Unit cell angles | 90.00, 101.43, 90.00 |
Refinement procedure
| Resolution | 48.030 - 2.100 |
| R-factor | 0.2156 |
| Rwork | 0.214 |
| R-free | 0.25264 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k7x |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.597 |
| Data reduction software | AutoProcess |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.110 | 0.480 |
| Number of reflections | 52497 | |
| <I/σ(I)> | 10.4 | 3.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | BT3792 AT A 20 MG PER ML BUFFERED IN IN 25 MM TRIS-HCL (PH 8.0), 500 MM NACL, 2 MM DTT WITH A RESERVOIR SOLUTION CONSISTING OF 5% GLYCEROL (V/V), 24% (W PER V) POLYETHYLENE GLYCOL 2,000 MONOMETHYL ETHER, 0.25 M SODIUM ACETATE, AND BIS-TRIS-HCL (PH 5.5) |
