4C1R
Bacteroides thetaiotaomicron VPI-5482 mannosyl-6-phosphatase Bt3783
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-06 |
| Detector | MARRESEARCH MX-300HE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.440, 148.810, 71.450 |
| Unit cell angles | 90.00, 117.10, 90.00 |
Refinement procedure
| Resolution | 39.150 - 2.100 |
| R-factor | 0.1582 |
| Rwork | 0.156 |
| R-free | 0.20495 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mpr |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.529 |
| Data reduction software | AutoProcess |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.120 | 0.440 |
| Number of reflections | 70728 | |
| <I/σ(I)> | 7.8 | 3.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | BT3783 CRYSTALS WERE OBTAINED BY MIXING EQUAL VOLUMES OF PURIFIED, RECOMBINANT BT3783 PROTEIN AT A CONCENTRATION OF 11.25 MG PER ML WITH MOTHER LIQUOR SOLUTION CONSISTING OF 15% (W/V) POLYTHEYLENE GLYCOL 4,000, 5% MPD (V PER V), 3 MM D-MANNOSE, AND 200 MM MGCL2., pH 8.0 |
